goat polyclonal igg anti sox2 antibody  (R&D Systems)

Journal: Cell Stem Cell

Article Title: Amniogenesis occurs in two independent waves in primates

doi: 10.1016/j.stem.2022.03.014

Figure Lengend Snippet:

Article Snippet: The following primary antibodies and dilutions were used: polyclonal goat IgG anti-human SOX2 (AF2018, RnD Bio-Techne) 1:500; polyclonal rabbit IgG anti-human CDX2 (3977S, Cell Signaling Technology) 1:200; monoclonal rabbit IgG anti-human GATA3 (ab199428, Abcam) 1:200; polyclonal goat IgG anti-human E-cadherin (AF748, RnD Bio-Techne) 1:100; monoclonal mouse IgG2b anti-human POU5F1 (sc-5279, Santa Cruz) 1:100.

Techniques: Recombinant, In Vitro, Cell Culture, Derivative Assay, Software

Journal: Cancer Microenvironment

Article Title: Targeting Hyaluronan Interactions for Glioblastoma Stem Cell Therapy

doi: 10.1007/s12307-019-00224-2

Figure Lengend Snippet: At baseline, GSC markers, HA receptors, and drug resistance genes are highly co-expressed in GBM, with cell-line dependence. a Percent of population expressing SOX2 and CD44 within U87-MG and D456 cells treated in NBE with varying concentrations of HA for a full passage length, measured via flow cytometry (mean ± SE; n = 3). b Confocal microscopy was performed on D456 cells grown in NBE for NANOG, SOX2, and HYAL1 (scale bars = 200 μm). c qRT-PCR gene expression of (left to right) GSC markers, HA-related genes, and drug resistance genes in U87-MG and D456 cells. Reported as relative expression compared to housekeeping gene GAPDH with log10 transformation and significance noted between cell lines. HYAL1 and MDR1 were lower than detection level (n.d.) in D456 (mean ± SE; n = 3; * p < 0.05, † p < 0.01, and ‡ p < 0.001

Article Snippet: Pellets were then stained with rabbit anti-SOX2 IgG polyclonal primary antibody (Proteintech) and goat anti-rabbit IgG Alexa Fluor 488 polyclonal secondary antibody (EMD Millipore).

Techniques: Expressing, Flow Cytometry, Confocal Microscopy, Quantitative RT-PCR, Transformation Assay

Journal: Cancer Microenvironment

Article Title: Targeting Hyaluronan Interactions for Glioblastoma Stem Cell Therapy

doi: 10.1007/s12307-019-00224-2

Figure Lengend Snippet: HAase decreases stemness through involvement of CD44 signaling. qRT-PCR gene expression of U87-MG and D456 cells treated with HAase for a full passage length. a GSC markers CD133, SOX2, NES, and NANOG. b HA-related genes HAS2, RHAMM, and CD44. c Drug resistance genes STAT3, EGFR, and MDR1. MDR1 was not detected in D456 cells. All genes reported as relative expression compared to housekeeping gene GAPDH (mean ± SE; n = 3;* p < 0.05, † p < 0.01)

Article Snippet: Pellets were then stained with rabbit anti-SOX2 IgG polyclonal primary antibody (Proteintech) and goat anti-rabbit IgG Alexa Fluor 488 polyclonal secondary antibody (EMD Millipore).

Techniques: Quantitative RT-PCR, Expressing

Journal: Scientific Reports

Article Title: Reduced expression of the murine HLA-G homolog Qa-2 is associated with malignancy, epithelial-mesenchymal transition and stemness in breast cancer cells

doi: 10.1038/s41598-017-06528-x

Figure Lengend Snippet: Pharmacological inhibition of Src reduces stemness. ( A ) Western blot analysis of Sox2 expression in 4T1m and 4T1t cells untreated or treated with PP2. Due to the high background observed in the blot, a positive control with a lysate of 293 T cells overexpressing Sox2 (c) was included. β-actin was used as a control for protein loading. ( B ) Flow cytometry analysis of the cell-surface markers CD44 and CD24 in 4T1m and 4T1t cells untreated or treated with PP2. The percentage of CD44 high /CD24 med/low cell subpopulation is indicated. A representative experiment out of two is shown.

Article Snippet: Brugge (Harvard Medical School, USA) and polyclonal Ab recognizing Src phosphorylated Y418 (1:1000) was from Biosource Int. (Invitrogen); polyclonal Ab for Twist1/2 (1:1000) was from Genentech; mAb AC-74 (1:10000) for β-actin from Sigma-Aldrich; mAb KM-201 (1:200) for CD44 was a kind gift from Dr. H. Yarwood (Imperial College London, UK); and polyclonal goat IgG for Sox2 (AF2018, R&D Systems), a generous gift from Dr.

Techniques: Inhibition, Western Blot, Expressing, Positive Control, Control, Flow Cytometry